Topo ta cloning kit invitrogen

The TOPO TA Cloning Kits for Sequencing provide a highly efficient 5-minute one-step cloning strategy TOPO Cloning for the direct insertion of Taq polymeraseamplified PCR products into a plasmid vector for sequencing. A0 Introduction Follow these instructions to use the TOPO TA Cloning Kit to clone your PCR product into the TOPO vector and transform.

Each kit contains pBAD TOPO TA Cloning reagents Box 1 One Shot TOP10 Chemically Competent E.

Topo ta cloning kit invitrogen. TOPO TA Cloning Kit From Invitrogen. A quick and easy kit for cloning PCR-generated products. The smallest kit available of 20 reactions.

For infrequent users a 10 reaction kit would be a useful option. A useful kit for researchers wanting to quickly and efficiently clone PCR products. Invitrogen TOPO TA Cloning Kit for Sequencing without competent cells.

Provides a highly efficient 5-minute one-step cloning strategy TOPO Cloning for the direct insertion of Taq polymeraseamplified PCR products into a plasmid vector for sequencing. Invitrogen TOPO TA Cloning Kits are designed for cloning PCR products amplified by Taq DNA polymerase Helpful tips which leaves an adenine at the 3 end of the product creating overhangs or sticky ends Figure 2. Invitrogen TOPO TA Cloning Kit for Subcloning with One Shot TOP10 chemically competent E.

Coli cells Provides a highly efficient 5-minute one-step cloning strategy TOPO Cloning for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning. The pCR 21-TOPO TA vector used in this kit comes with 3-T overhangs for efficient ligation of Taq polymeraseamplified PCR products which contain 3-A overhangs. The standard in cloning When it comes to cloning TOPO cloning technology has been a reliable partner for thousands of scientists for over ten years.

Fast simple-to-use and. Invitrogen TOPO PCR cloning technology was developed to help improve cloning efficiency simplify protocol setup and accommodate a wide range of PCR insert sizes. TOPO technology enables inserts with compatible ends to be readily joined to the vector in 5.

Select TOPO TA Cloning Kits are also available with PureLink Quick Plasmid Miniprep Kit. One Shot Cells Type of Cells Reactions TOPO TA Cloning Kit with pCR 21-TOPO vector K4500-01. The TOPO TA Cloning Kits for Sequencing provide a highly efficient 5-minute one-step cloning strategy TOPO Cloning for the direct insertion of Taq polymeraseamplified PCR products into a plasmid vector for sequencing.

Each kit uses the pCR4-TOPO TA vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each. Quick and efficient PCR cloning with TOPO TA cloning. Clone PCR-amplified DNA fragments blunt or A-overhang directly into a choice of over 40 subcloning sequencing or expression vectors in just 5 minutesand obtain up to 95 recombinant clones.

Quick avoid inefficient ligation and laborious searches for appropriate restriction enzymes. TOPO TA Cloning Dual Promoter kits are for fast efficient cloning and subsequent in vitro transcription. These kits include the pCRII-TOPO TA vector with dual T7 and SP6 promoters.

By eliminating time-consuming and tedious restriction site cloning TOPO cloning is the most reliable method featurin. The TA Cloning Kit uses the pCR21 cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteen-minute room-temperature ligation step. Reactions typically yield 80 recombinants containing inserts.

Bluewhite screening and 80 clones with. Includes the pCRII-TOPO TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning TOPO cloning is the most reliable cloning method featuring a 5-minute cloning reaction and 3-step protocol and yielding up to 95 recombinants.

The TOPO TA Cloning Kits for Sequencing are shipped on dry ice. Kits containing competent cells include box with TOPO TA Cloning Reagents Box 1 and a box with One Shot competent. TOPO TA Cloning Kit for Sequencing supplied with the PureLink Quick Plasmid Miniprep Cat.

NoK4575-02 is shipped with an. The TOPO TA Cloning Kit is shipped on dry ice. Each kit contains a box with TOPO TA Cloning reagents Box 1 and a box with TOP10 or TOP10F One Shot competent cells Box 2.

Store Box 1 at -20C and Box 2 at -80C. Types of TOPO TA Cloning Kits Two different types of TOPO TA Cloning Kits are available in two different. TOPO TA Cloning Kits Catalog Numbers K4500-01SC K4550-01SC K4600-01SC Doc.

A0 Introduction Follow these instructions to use the TOPO TA Cloning Kit to clone your PCR product into the TOPO vector and transform. Iv Kit Contents and Storage Shipping and Storage The pcDNA31V5-His TOPO TA Expression Kit is shipped on dry ice. Each kit contains a box with pcDNA31V5-His TOPO TA Cloning reagents Box 1 and a box with One Shot TOP10 chemically competent cells Box 2.

Store Box 1 at -20C and Box 2 at -80C. Types of Kits Ordering information for the pcDNA31V5-His TOPO TA Expression. Learn how simple it is to get 95 recovery of yo.

Invitrogen TOPO TA Cloning Kit for Subcloning without competent cells Brand. Invitrogen 450641 31372 EUR valid until 2021-12-31 Use promo code 14807 to get your promotional price. The TA Cloning Kits are shipped on dry ice and contain a box of TA Cloning Reagents Box 1 and a box of One Shot Competent Cells Box 2.

K2020-20 and K2020-40 are not supplied with One Shot Competent Cells. Store Box 1 at 30C to 10C in a. 4 Kit Contents and Storage Types of kits The pOptiVEC -TOPO TA Vector Kit is included with the FreedomDG44Kit and as a separate catalog item.

Number Freedom DG44 Kit A13737-01 pOptiVEC-TOPO TA Vector Kit 12744-017 Shipping and storage The pOptiVEC-TOPO TA Vector Kit is shipped on dry iceEach kit contains two. The TOPO TA cloning method combines the advantages of TA cloning with the ligation activity of topoisomerase I. This allows direct ligation of PCR products in just 5 minutes.

TOPO TA cloning kits are available from Invitrogen. The pBAD TOPO TA Expression Kit is shipped on dry ice. Each kit contains pBAD TOPO TA Cloning reagents Box 1 One Shot TOP10 Chemically Competent E.

Coli Box 2 and a small bag with an LMG194 stab. Store Box 1 at 20C and Box 2 at 80C. Store the LMG194 stab at 4C.

TOPO TA Cloning Reagents.