Reasons for identifying unknown bacteria

MAC is also differential in identifying gram-negative lactose. In research it is a must to be able to identify unknown bacteria if you are comparing the different microorganisms.

The second test performed was a Urea Test which also gave a clear positive result confirming that unknown gram positive bacteria as Staphylococcus epidermidis because Staphylococcus aureus gives a negative result.

Reasons for identifying unknown bacteria. WHY IS THE IDENTIFICATION OF BACTERIAL UNKNOWNS IMPORTANT. Microbiologists must identify bacterial isolates for several practical reasons. Medical diagnostics identifying a pathogen isolated from a patient.

Food industry identifying a microbial contaminant responsible for food spoilage. Unknown Bacteria Unknown bacteria determined to be Alcaligenes faecalis because of its morphological physiological and metabolic properties. In a laboratory setting it often becomes necessary to identify an unknown organism.

Unknown bacteria are first collected by swabbing surfaces around and near the lab then streaked on sterile LB agar plates and grown overnight in an incubator. A different unknown number is given to each place bacteria are collected. Students then use these plates to make their own unknown plate by streaking for single colonies.

The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others make antibiotics used today.

Identifying the Bacterium If you have an unknown bacteria and you want to identify it youll typically perform a gram stain and then observe the colony appearance and the individual features. At that point you can say you have for example a gram-negative aerobic streptobacilli. Selective and differential media are used to isolate and identify specific organisms.

In this experiment that used different methods and tests unknown bacteria HJ were isolated and identified. Staining is used to color and separate bacteria. Each pair will receive one unknown organism to identify.

You will conduct tests appropriate for your organism to determine genus and species identification. Each pair may have to present information on the specific organism they identified including. Test results where that organism is part of the normal flora when and where that organism becomes a pathogen possible diseases the organism causes.

Some bacteria are parasites that survive in the animals man and in some plants causing diseases. The various diseases that are caused by the bacteria due to the release of the toxins by them inside the cells made it necessary to identify the bacteria. Reasons range from the knowing the causative agent of a disease in a patient so as to know how it can be treated to knowing the correct microorganism to be used for making certain foods or antibiotics.

Techniques for Identifying Unknown Bacteria. 3234 words 13. Present in growth culture can lead to misinterpretation of results and ultimately sabotage the identification of the unknown bacteria for this reason using the aseptic method is the most crucial method of culture transfer.

The unknown 118 was given to which a series of. Importance Of Identification Of Unknown Organisms. Identification of unknown organisms is useful in a wide variety of settings.

From clinical diagnostics to environmental monitoring 1. Species of bacteria can differ fundamentally in aspects ranging from morphology to metabolism. The Identification of Two Unknown Species of Bacteria in Tube 72 Introduction.

There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient so as to know how it can be treated to knowing the correct microorganism to be used for making certain foods or antibiotics. Identification of unknown bacteria Identifying microbes is important for a number of reasons.

In food microbiology identification of food spoilage microbes can give direction towards intervention to prevent further corruption of the food supply or food processing. In medical microbiology it may yield very important information about new sources or routes of infection again with the goal of. The strategy for identifying unknowns is to eliminate all bacteria giving contradictory results to those found in the Identification Matrix.

This is done automatically when you input results. Input a result and all bacteria in the matrix with a - result are eliminated from consideration. The identification of bacteria is a careful and systematic process that uses many different techniques to narrow down the types of bacteria that are present in an unknown bacterial culture.

It produces benefits for many aspects of the research of microorganisms and helps physicians correctly treat patients. Identification of an Unknown Bacterium and Writing Up a Report A standard part of nearly all lab courses in introductory microbiology is an activity wherein the student must use everything that has been learned in the course to identify and unknown bacterial culture. My unknown can either be any of the following gram bacteria.

Bacillus Cereus Bacillus Subtilis Stapahylococcus Aureus Staphylococcus Epidermidis or Enterococcus faecalisMannitolTo determine bacteria that grows in high salt concentrationMannitol agarRed agar mannitol turns yellow colorUreaTo determine if bacteria can detect ureaseUrea brothNo change in color. The second test performed was a Urea Test which also gave a clear positive result confirming that unknown gram positive bacteria as Staphylococcus epidermidis because Staphylococcus aureus gives a negative result. Consultation with the Professor confirmed the two unknown bacteria correctly as Pseudomonas aeruginosa and Staphylococcus epidermidis.

The only problem that was. Identifying the bacteria is crucial when treating a bacterial infection because the correct antimicrobial or antibiotic must be used to successfully eliminate the infection and this cannot be done without the identity of the bacteria. Selecting the correct antibiotic is critical when dealing with an infection since not all antibiotics are equally effective against bacteria.

The unknown gram-negative bacterium was identified as Escherichia coli. MacConkey Agar was first used to isolate the gram-negative bacterium because it inhibits the growth of gram-positive bacteria. MAC is also differential in identifying gram-negative lactose.

Coli strands are non pathogenic however there are strands known to cause food poisoning. Introduction There are multiple reasons for identifying unknown bacteria. In research it is a must to be able to identify unknown bacteria if you are comparing the different microorganisms.

This biochemical test checks the bacterias ability to hydrolyze gelatin by secreting a proteolytic enzyme called Gelatinase Harley 167 To inoculate the tube of nutrient gelatin one must stab the media with an inoculating needle that contains a portion of the Unknown using aseptic techniques. If the bacterium is positive for Gelatinase activity the media in the inoculated tube will no. Identification of Unknown Bacteria.

Help save baby Kuppelfangs from an epidemic. Visit the exoplanet Astakos IV where the Kuppelfang population is suffering from a bacterial infection. Use staining differential media and biochemical tests to identify the bacteria so that the Kuppelfangs can be treated before they go extinct.