Microscope lab using the microscope and slide preparation

Use a microscope with x100 magnification and an eyepiece graticule to observe the slides over the next 15-30 minutes for signs of pollen germination. When finished using the microscope complete the following.

Use the microscope to focus on the slide - try different objectives some may.

Microscope lab using the microscope and slide preparation. The best specimens usually come from the bottom and probably will contain chunks of algae or other debris that you can see with your naked eye. Be careful that your slide isnt too thick 2. Use the microscope to focus on the slide - try different objectives some may.

Be careful that your slide isnt too thick 2. Use the microscope to focus on the slide - try different objectives some may be better than others for viewing the slide. Make three separate drawings below at different areas of the slide and at different magnifications.

Prepare a wet mount of pond water - a sample of pond water is provided in a jar. The best specimens usually come from the bottom and probably will contain chunks of algae or other debris that you can see with your naked eye. Be careful that 2.

Use the microscope to focus on the slide -. Be careful that your slide isnt too thick 2. Use the microscope to focus on the slide - try different objectives some may be better than others for viewing the slide.

Make three separate drawings below at different areas of the slide and at different magnifications. View Lab Report - Microscope Lab Using the Microscope and Slide Preparationdocx from SCIENCE 3250 at Lowell High Lowell. Microscope Lab Using the Microscope and Slide Preparation.

Examine the microscope and familiarize yourself with the parts of the microscope. The magnification written on the ocular lens eyepiece is _____________. The magnification on the Scanning objective ___________ Low Power Objective ___________.

Although wet mounts can be used to prepare a significantly wide range of microscope slides they provide a transitory window as the liquid will dehydrate and living specimens will die. Organisms such as protozoa may only live 30 minutes under a wet mount slide. Applying petroleum jelly to the outer edges of the cover slip creates a seal that may extend the life of the slide up to a few days.

Use a microscope with x100 magnification and an eyepiece graticule to observe the slides over the next 15-30 minutes for signs of pollen germination. Between quick observations turn the microscope lamp off and return the slide to the Petri dish. Pollen grain are seen with the pollen tubes.

Because of the microscopy requirements options for preparing specimens are limited to. Whole-mounts where an entire organism or structure is small enough or thin enough to be placed directly onto a microscope slide eg a small unicellular or multicellular organism or a membrane that can be stretched thinly on to a slide. Obtain a clean microscope slide with a frosted edge.

Label the frosted edge appropriately with the sample identification. Transfer specimen or culture to the center of the slide. Prepare a thin layer of cells on the slide.

Refer to your laboratorys procedure according to different specimen types. View Kami Export - Delaney McNickle - Microscope Labpdf from BIO 101 at Perry High School. Microscope Lab - Using the Microscope and Slide Preparation Name.

Turn on the light source. Put the slide on the specimen stage held by the slide clip. Select the objective the part containing the lens with the lowest magnification.

Center the specimen over the circle of light. Use the coarse focus knob first before using the fine focus knob. Microscope always hold the arm while supporting the base with your other hand.

Proper Use of the Compound Light Microscope Exercise 1B Steps to follow when using the microscope If you really want to be able to see a specimen on a slide you must follow the steps on the next page every time you look at a new slide. The microscope will be your friend if you always use the following. Make your own prepared slide with mounts of your choice of specimen on glass microscope slides.

This is a great microscope activity for junior high to high school age. Or make simple slides out of household items a project that works well for elementary age kids and can be. The preparation of a microscopic slide may take hours and for those who have no experience the slide may not be well prepared for viewing which is time wasted.

Moreover the prepared microscope slide set is composed of a good number of preparations given that it contains 50 to 100 slides. Take a piece of microscope slide and place a drop of the specimen onto the slide. Using a second piece of a glass slide smear the specimen around the slide by dragging the smearing slide across the first.

You can make a longer smear by smearing at a low angle and a thicker smear by backing the smearing slide or using two drops of the specimen. When finished using the microscope complete the following. Check off each step when completed.

Bring the stage to its lowest level. Click the 4X objective into place. Remove the slide and dispose of appropriately.

Wipe prepared slides until ALL oildirt is. Place your prepared slides next to each other on a paper towel or staining rack. Cover the slides in the chosen stain and leave for 1-2 minutes.

Remove excess stain by running a gentle stream of water over the surface of the glass slide. Place the glass slide on the microscope stage and begin viewing the specimen. Scan the slide for organisms.

Start at the upper left-hand corner and move the slide forward and back as you work across the slide from left to right. Experiment by using all available lenses NOT the oil immersion by focusing up and down with the fine-adjustment knob. This lab is designed to examine the structure of an animal cell using a microscope.

Students will make their own wet mount slide using tape instead of swabbing their mouth so that it is a bit more sanitary. Students will also practice their drawings by observing various prepared slides. Now using the skill of constructing a wet mount follow the steps above to help you.

1 Set up your microscope at your workstation. 2 Prepare your first wet mount. When it is complete look at it under the microscope.

Draw what you see. 3 Now prepare a wet mount with too much water. Draw what you see and make.

To prepare a wet mount microscope slide simply place a few drops of the required liquid on the slide using the dropper before placing the specimen in the centre of the slide. Next add a few more drops of liquid until the specimen is covered. This will reduce the.

Wet slide will stick on the stage of the microscope Sit so that the arm of the microscope is closest to you and place the slide on the stage with the e in a normal reading position for you.