23
Jul
The purpose of this lecture is to introduce you to terminology used in microbiology. In the clinical microbiology laboratory where many traditional.
If the answer to a question is yes then do X.
Microbiology unknown identification chart. Unknown Identification Work Flow Flowchart Author. TMCC Biology Department Microbiology Resource Center Created Date. The first thing to prepare for an Unknown Identification exercise is to make a dichotomous key.
Keys are charts that require decisions at branch points much like a flow chart in computer logic. If the answer to a question is yes then do X. If the answer is no then do Y.
Flowchart For First Unknown Identification Gram Stain Negative See Reverse Side Positive 20 Mycobacterium Tis Rods Cocci Course Hero Flow Chart Microbiology Lab Day 1 Unknown 30 Received Was Streaked On Two Agar Plates Incubated At 37c And Room Temperature 2. Microbiology flowchart unknown bacteria Microbes can make a big difference. Bacteria can communicate with each other and coordinate their actions.
Although most bacteria are produced with single microscopic cells they are not as easy as previously believed. Each pair will receive one unknown organism to identify. You will conduct tests appropriate for your organism to determine genus and species identification.
Each pair may have to present information on the specific organism they identified including. Test results where that organism is part of the normal flora when and where that organism becomes a pathogen possible diseases the organism causes. Receive your unknown tube and immediately record its code label in your notebook.
OU stands for the Other Unknown. Label the unknown tube with your initials. Do not let the tube stand at room temperature for long.
After finishing your work return the tube to the refrigerator as soon as possible. Table I and Flowchart I list all the tests purposes results and order the tests where done for the Gram positive bacteria using the Unknown 109 tube. Table II and Flowchart II list all the tests purposes results and order the tests where done for the Gram negative bacteria using the Alternative 4 tube.
By using both the Basic Bacteria Identification diagrams and the Table of Bacterial Causes of Infection following diagrams you can identify the likely bacteria from the Gram film appearance on the microscopy result. The patient clinically has meningitis. The Gram film of the CSF shows Gram-positive cocci in chains.
Learn microbiology lab identification unknowns with free interactive flashcards. Choose from 500 different sets of microbiology lab identification unknowns flashcards on Quizlet. The purpose of this lecture is to introduce you to terminology used in microbiology.
Cover different classification schemes for grouping bacteria especially the use of the Gram stain 2. Describe the different types of bacteria 3. Discuss bacterial structure and the function of the different bacterial components 4.
Use Createlys easy online diagram editor to edit this diagram collaborate with others and export results to multiple image formats. You can edit this template and create your own diagram. Creately diagrams can be exported and added to Word PPT powerpoint Excel Visio or.
A different unknown number is given to each place bacteria are collected. Students then use these plates to make their own unknown plate by streaking for single colonies. In the first step in the biochemical identification students use a single colony to streak an EMB-lactose agar plate to determine if their unknown is gram.
The unknown gram-negative bacterium was identified as Escherichia coli. MacConkey Agar was first used to isolate the gram-negative bacterium because it inhibits the growth of gram-positive bacteria. MAC is also differential in identifying gram-negative lactose fermenters that produce a pink color.
Microbiology - Unknown Identification Tests. Microorganisms can be identified by their _______ which diges. Starch hydrolysis indicates the presence of this exoenzyme.
Lipid hydrolysis indicates the presence of this exoenzyme. Casein hydrolysis indicates the presence of this exoenzyme. Microbiology lab flow chart for gram staining and cocci species single amino acid utilization for bacterial categorization scientific reports anaerobic identification flowchart using minimal laboratory resources antibos and immune effectors shaping gram negative bacterial phenotypes trends in microbiology biology 41 microbiology unknown check.
Microbiology 20 Biochemical Unknown Spring 2009 due May 14th You should be prepared to turn in your notebook with your biochemical unknown identification completed after lab on Thursday May 14th. Out of a possible 70 points you will be graded as follows. Successfully performing the necessary tests to identify your unknown 25 pts.
1 Inoculate 2 Different TSA Agar Slants Using Single Line to Get a Growth Pattern. 2 Inoculate 3 Different TSB Broth Tubes Using 3 Loop-Fulls of Your Unknown to Determine the Growth Pattern in Broth and to Determine the Optimal Growth Temperature. You will incubate the broth at different temperatures to determine the optimum growth.
Microbiology aid for unknown identification flow charts manual of determinative bacteriology all of the unknowns will fall into the following groups in manual. The unknown 123 contained two different specimen of bacteria one being a gram positive bacteria and one being a gram negative bacteria. The first unknown in 123 found to be a gram negative bacteria was identified as Pseudomonas aeruginosa.
This identification was reached by only one biochemical test and close observation. MODULE Bacterial Identification Tests Microbiology 130 Notes Procedure Christensens Urea Agar 4 5 Use a heavy inoculum from an 18- to 24-hour pure culture to streak the entire slant surface. Do not stab the butt as it will serve as a color control.
To identify the bacterial unknowns in a mixed culture by morphological and biochemical methods. The identification of bacteria is a careful and systematic process that uses many different techniques to narrow down the types of bacteria. HttporegonstateedudeptmicrobiologywritingmanualWIC_WritingManualpdf SOME ADDITIONAL POINTS.
Attach both the working worksheet and the final worksheet 2. Attach the unknown identification table that was used for identifying the unknown with the marks used to help narrow down results. Number the pages 4.
Use a spell checker 5. UNKNOWN LAB REPORT. The study of microbiology requires not only an academic understanding of the microscopic world but also a practical understanding of lab techniques and procedures used to identify control and manipulate microorganisms.
A guiding principle of clinical laboratories is to provide patient test results as quickly as possible while maintaining accuracy and quality. Therefore advancements in analytical technology that not only improve accuracy and quality but also decrease the length of time from sample collection to results are highly desirable. In the clinical microbiology laboratory where many traditional.